TABLE 4.
Localization of Fts proteins in FtsEX depletion backgrounda
GFP fusion | Sugar | No. of cells scored | Avg cell length (μm) | % of cells with the indicated no. of rings
|
Spacing of ringsb | |||||
---|---|---|---|---|---|---|---|---|---|---|
0 | 1 | 2 | 3 | 4 | 5 | |||||
FtsA-GFP | Arabinose | 164 | 4.8 | 7 | 85 | 8 | 0 | 0 | 0 | 3.6 |
Glucose | 93 | 35 | 0 | 7 | 23 | 13 | 23 | 34 | 7.3 | |
ZipA-GFP | Arabinose | 224 | 4.1 | 20 | 79 | 1 | 0 | 0 | 0 | 5.0 |
Glucose | 112 | 28 | 4 | 10 | 35 | 19 | 19 | 13 | 9.5 | |
FtsK(1-266)-GFP | Arabinose | 243 | 6.3 | 49 | 51 | 0 | 0 | 0 | 0 | 12 |
Glucose | 101 | 21 | 67 | 29c | 4c | 0 | 0 | 0 | 55 | |
GFP-FtsQ | Arabinose | 217 | 7.2 | 35 | 65 | 0 | 0 | 0 | 0 | 11 |
Glucose | 131 | 24 | 69 | 27c | 4c | 0 | 0 | 0 | 67 | |
GFP-FtsI | Arabinose | 108 | 5.0 | 63 | 37 | 0 | 0 | 0 | 0 | 14 |
Glucose | 66 | 23 | 68 | 32c | 0 | 0 | 0 | 0 | 72 |
Strains used were EC1363, EC1391, EC1386, EC1392, and EC1366.
See Table 3, footnote b.
These bands were faint, and so the numbers given understate the degree of dependence on FtsEX.