Fig. 3.

Bars: means ± SEM of the number of rats indicated at the bottom. a LPS injected intraperitoneally increases plasma CRH levels after 6 h. Rats were injected intraperitoneally with LPS (100 μg/kg body weight) or vehicle (saline) and decapitated at the time points indicated at the x-axis. Trunk blood was collected and processed according to the RAPID method. ∗ p < 0.05 compared to vehicle. b Mean CRH tissue levels in the proximal colon show a trend towards a decrease at 6 h after LPS injection. Tissue of the proximal and distal colon was harvested at 6 h after LPS or vehicle injection, and total protein was extracted for RIA. ns = Not significant.