FIG. 1.

Primer extension analyses of transcripts originating from the betL promoter. Cells were grown in BHI (OD₆₀₀, ∼0.5), harvested, washed, and resuspended in 50 mM phosphate potassium buffer with 50 mM glucose. The washed cell suspension from each strain was divided into two equal volumes, and NaCl was added to a 3% final concentration in one portion. After a 20-min incubation, RNA was harvested from the cells, and 50 μg of each RNA sample was used as template for extension of the labeled PEX3 primer. Strain designations are as follows: 10403S (WT); 10403S plus 3% NaCl (WT+S); LMA2B (sigB::Kan) (sigB); LMA2B plus 3% NaCl (sigB + S). The sequence ladder was derived from reactions using the PEX3 primer, and the top strand sequence is shown to the right of the ladder (reverse complement of the sequence ladder). The nucleotide corresponding to the 3′ end of the extension product is underlined. The corresponding top strand sequence is shown below, with the +1, −10, and −35 regions underlined.