FIG. 8.
Promoter alignments. (A) Alignment of known σB-dependent promoters. The sequences immediately upstream of the σB-dependent transcription start site of the L. monocytogenes rsbV gene, opuC, and gbuA are aligned with several σB-dependent promoters from B. subtilis. The most highly conserved residues centering on the −10 and −35 regions are boxed. The alignment of B. subtilis promoters was derived from Petershon et al. (24), Helmann et al. (16), Scharf et al. (25), Antelmann et al. (4), Akbar et al. (1) and von Blohn et al. (33). Bs, B. subtilis; Lm, L. monocytogenes. (B) Alignments of the betL and gbuAP1 promoters. The sequences upstream of the start sites of betL and gbuA are aligned, and the most highly conserved residues centering on the −10 and −35 regions are boxed.