Skip to main content
. 2011 Feb 5;54(6):339–348. doi: 10.1159/000321452

Fig. 4.

Fig. 4

Inhibitors of EGFR or ERK in bile acid-mediated promotion of HCV replication. Semiconfluent GS4.1 or 1A7 cells were incubated with mock medium, CDCA, AG1478 or CDCA + AG1478 for 24 h, and HCV replication was measured by detecting HCV RNA (a, b) or protein (c). a Real-time qRT-PCR analysis of HCV RNA levels after the treatment with CDCA 100 μM (CDCA100), AG1478 30 μM (AG30), CDCA100 + AG30, or CDCA100 + AG1478 15 μM (AG15). b Real-time qRTPCR analysis of HCV RNA levels after the treatment with CDCA100, U0126 20 μM (U20), CDCA100 + U20, or CDCA100 + U0126 10 μM (U10). Asterisk (∗) indicates that the RNA levels by double treatment with CDCA and AG1478 or U0126 were significantly reduced compared to those by the treatment with CDCA alone (p < 0.05). c Western blot analysis of HCV protein NS5B in GS4.1 cells after treatment with CDCA, AG1478 or CDCA + AG1478. Lane 1: Mock; lane 2: CDCA 100 μM; lane 3: AG1478 30 μM + CDCA 100 μM; lane 4: AG1478 5 μM + CDCA 100 μM, and lane 5: AG1478 30 μM. As a loading control, Western blot analysis of β-actin was performed with the same samples.