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. 2004 Feb;186(3):889–893. doi: 10.1128/JB.186.3.889-893.2004

FIG. 2.

FIG. 2.

Biofilm formation for V. vulnificus strains under different growth conditions. Strains of V. vulnificus, including MO6-24/O, MO6-24/T, MO6-24/31T, and CVD752, were examined for biofilm formation in microtiter plates in LB prepared with either 1% NaCl, pH 7 (A), 2% NaCl, pH 7 (B), 1% NaCl, pH 8 (C), or 2% NaCl, pH 8 (D) at incubation temperatures of 30 and 37°C as indicated. The optical density at 540 nm of eluted dye from attached cells is indicative of relative bacterial cell concentration in biofilms. Significant differences (P < 0.05) in destained biofilms between MO6-24/O and other strains (*) and between MO6-24/T and acapsular mutants (#) are noted.