FIG. 1.

Disulfide isomerase activities in mutant strains. Cells were grown in rich medium as described in Materials and Methods, harvested by centrifugation, and lysed with a French pressure cell. Equal amounts of cell lysate protein were used to determine formation of active tPA, as measured by the fibrin clearance assay (A), tPA activity, as determined by a coupled chromogenic assay that monitored the rate of conversion of plasminogen to plasmin (B), and accumulation of folded BPTI, as monitored by an enzyme-linked immunosorbent assay (C). In panels B and C the data are averages of three experiments. WT, wild type.