Figure 3.

Dual localization of Usb1p. Cells expressing the Usb1-GFP and Nic96-CFP fusion proteins were grown in complete glucose medium with a five times excess of adenine at 28°C, washed once and incubated with Mitotracker red CMX ROS (MT, Molecular Probes) for 10 min. After two additional washes, cells were observed by fluorescence microscopy with the appropriate filters to visualize GFP, CFP or Mitotracker.