Figure 3.

Zac1 is required for HDAC inhibitor-induced NF-κB activity repression. (a) Cells were co-transfected with 0.5 μg of 3 × κB-Luc reporters and the same amount of Renilla luciferase reporter plasmid. Twenty-four hours after transfection, cells were stimulated with the indicated concentration of TSA or NaBt. Luciferase activity was measured 12 h after treatment. Luciferase activity was presented relative to Renilla luciferase activity. (b) 3 × κB-Luc and Renilla luciferase reporters were co-transfected into different F9 and P19 cell clones as indicated. Twenty-four hours later, cells were treated with 20 ng/ml TSA or 1.5 mM NaBt for 12 h. Vehicle-treated cells were used as controls. Cells were subjected to luciferase activity assay. (c) F9 and P19 cell clones were treated with 20 ng/ml TSA for 24 h. Then the mRNA levels of the indicated genes were analyzed by RT-PCR