Figure 7.

The effect of HDAC inhibitors on several non-cancer cell lines. (a) Cells were treated with 50 ng/ml TSA (left) or 5 mM NaBt (right) for 48 h. Cell apoptosis was determined by FACS assay. (b) Cells were treated with the indicated concentration of TSA for 24 h. Then the Zac1 transcriptional level was examined by RT-PCR. (c) Total RNA was extracted from the cells, and then the mRNA levels of the indicated genes were detected by RT-PCR. (d) Renilla luciferase and 3 × κB-Luc reporters were co-transfected into cells as indicated. Twenty-four hours later, cells were treated with TSA (left two panels) or NaBt (right two panels) for 12 h, followed by luciferase activity assay