Figure 3.

The different effects of different levels of transgenic IKKα on rescuing the skin phenotype of Ikkα^−/− mice and inducing keratinocyte terminal differentiation. (a) Transgenic IKKα rescued the skin phenotype of Ikkα^−/− mice at birth. +/+, Ikkα^+/+; −/−, Ikkα^−/−. (b) Histology of hematoxylin and eosin-stained skin sections from Tg-4/Ikkα^−/− (Tg-4/−/−), Ikkα^−/− (−/−), and Tg-7/Ikkα^−/− (Tg-7/−/−) newborns. Scale bars =150 μm. (c) Immunofluorescence staining of HA in the skin of WT (+/+), Tg-4/−/−, and Tg-7/−/− mice. Dotted lines mark the junction of the epidermis and the dermis. Scale bars=150 μm. (d) Levels of filaggrin in the skin of newborn mice, detected by western blotting. β-Actin was used as the control