Figure 7.

IKKα or reduction of EGFR represses inflammation pathways and skin thickness and inflammation. (a) IKKα inhibits ERK, JNK, c-Jun, and Stat3 activity in an IKKα dose-dependent manner in the skin. Western blotting examined indicated proteins in the skin specimens of WT (+/+), Tg-4, Tg-7, Ikkα^−/− (−/−), Tg-4/Ikkα^−/− (−/−+Tg-4), and Tg-7/Ikkα^−/− (−/−+Tg-7) newborns. p, phosphorylated; NS, non-specific bands; β-Actin, protein loading control. (b) Western blotting shows protein levels in WT, Ikkα^F/F/K5.Cre (F/F/K5.Cre), and Ikkα^F/F/K5.Cre/Egfr^+/− with 16-day-old mice. (c) Comparison of hematoxylin and eosin-stained skin histology of in WT (+/+), Ikkα^F/F/K5.Cre (F/F/K5.Cre), and Ikkα^F/F/K5.Cre/Egfr^+/− (F/F/K5.Cre/Egfr+/−) with 16-day-old mice. Scale bars=100 μm