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. 2011 Oct 13;4:407. doi: 10.1186/1756-0500-4-407

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Copyright ©2011 Bay-Jensen et al; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Western blot detection of CTR in human chondrocytes. (A) Screening of a panel of anti-CTR antibodies against human osteoclast lysates. A 53 kDa band (arrow), consistent with the receptor's molecular weight, was chosen to evaluate antibody performance. Ab1: H00000799-M01 diluted 1:200; Ab2: SP1338P dil. 1:200; Ab3: 250618 dil. 1:200; Ab4: Ab11042-50 dil. 1:2000. (B) Simultaneous detection of the 53 kDa CTR band in human osteoclasts and chondrocytes OCL: 10 μg and 20 μg protein of in vitro differentiated human osteoclasts lysates [34]; CHO1: 30 μg and 15 μg protein of chondrocytes lysates from the same individual; CHO2: 30 μg and 15 μg protein of chondrocytes lysates from a second individual. Antibody: Ab3, dil. 1:200. (C) Detection of CTR in 20 μg protein cell lysates samples of freshly isolated chondrocyte from two different individuals. Antibody: Ab4, dil. 1:2000.