TABLE 3.

Studies of holo-htf binding to wild-type and point-mutated MBP-TbpB using BIAcore and ITC analyses

MBP-TbpB	Holo-htf-binding capacity using BIAcorea	Dissociation constant using ITC (nM)b
Wild type	1,727 ± 182	126 ± 16
R55N	94 ± 12	345 ± 90
R55E	1 ± 2	NB
R80N	1,345 ± 162	172 ± 74
R243N	6 ± 5	NB

^aIn resonance units.

^bNB, no binding.