Fig. 1.

Flow cytometry enables basophil phenotyping from one drop of blood. a (1) Doublets were excluded based on forward scatter area versus height; (2) leukocytes were selected based on forward and side scatter; (3) dead cells were excluded using the viability marker Live/Dead Near InfraRed; (4) basophils were selected as the CD3–/CD16–/CD20–/CD56–/CD66b–/HLA-DR– and CD123+ population; (5) platelets were excluded using CD41a; (6) confirmation of gated basophil subset as intermediate for forward scatter and low for side scatter. b Basophil CD11b, CD63, CD203c and CD294 levels at baseline from 1 healthy control (HC) and 1 subject with peanut allergy.