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. Author manuscript; available in PMC: 2011 Dec 23.
Published in final edited form as: Nature. 2011 May 29;474(7352):511–515. doi: 10.1038/nature10085

Figure 4. Flt1 expression in myeloid cells is regulated by a Wnt pathway.

Figure 4

(a) PCR for Flt1 transcript in flow-sorted RMCs. (b) P14 retinal sections labeled for Flt1 and Iba1. 25 μm scale bars. (c) P18 vessel branch points in the superficial and deep vasculature in Flt1+/fl and Flt1+/fl; cfms-icre mice (n=7, Student’s T-test). n=7. (d) Isolectin labeling of the retinal vasculature in labeled genotypes. 50 μm scale bars. (e) PCR for indicated transcripts in E11.5 whole embryo (con) and RAW264.7 cells. (f, g) QPCR (f) for sFlt1 and mFlt1 in Wnt-treated RAW264.7 cells and ELISA (g) for sFlt1 on medium from Wnt-treated RAW264.7 cells (n=4, One Way ANOVA). (h) QPCR for sFlt1 in flow-sorted deep RMCs from Wls+/fl and Wls+/fl; cfms-icre mice (n=4, Student’s T-test). Errors are SEM. NS – Not significant.