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. 2011 Oct 26;108(45):18536–18541. doi: 10.1073/pnas.1111597108

Fig. 2.

Fig. 2.

EDC cross-linking in three types of purified PSII complexes. (A) SDS/PAGE analysis showing slower migration of His/Myc-tagged Psb27 (18 kDa, lane 1) than native Psb27 (11 kDa, lane 2) after Coomassie Brilliant Blue (R-250) staining. Lane 1: His27△ctpAPSII; lane 2: HT3△ctpAPSII; lane 3: HT3△ctpA△psb27PSII. Two micrograms of chlorophyll a-containing samples were loaded in each lane. (B) Detection of cross-linked species. Lane 1: His27△ctpAPSII; lane 2: His27△ctpAPSII + EDC; lane 3: HT3△ctpAPSII + EDC; lane 4: HT3△ctpA△psb27PSII + EDC. EDC (30 mM) cross-linking reaction was performed for 30 min in the dark at 23 °C. Immunoblot was probed with anti-Myc antibodies. (C) Same as in B except probed with anti-CP43 antibodies. Arrows indicate the increased mobility of CP43–Psb27 cross-linked complex (lane 3) compared with His/Myc-tagged Psb27–CP43 cross-linked complex (lane 2) and the absence of any cross-linked product in lane 4 because of the absence of Psb27 in HT3△ctpA△psb27PSII.