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. 2011 Oct 26;108(45):18536–18541. doi: 10.1073/pnas.1111597108

Fig. 3.

Fig. 3.

DTSSP cross-linking in His27△ctpAPSII preparation. (A) Lane 1: His27△ctpAPSII; lane 2: His27△ctpAPSII + DTSSP. The cross-linking reaction was performed by using 10 mM DTSSP for 30 min in the dark at 23 °C. Immunoblot was probed with anti-Myc antibodies. The location of the 58-kDa CP43–Psb27 cross-linked species is indicated with an arrow. (B) Same as in A except probed with anti-CP43 antibodies. (C) Results of 2D diagonal electrophoresis of His27△ctpAPSII complex treated with DTSSP and probed with anti-Myc antibodies. During SDS/PAGE, β-mercaptoethanol was omitted in the first dimension and was included in the second dimension. (D) Same as in C except probed with anti-CP43 antibodies. (E) Overlay of C and D. Protein spots that represent tagged Psb27 and CP43 are found as a vertical series below the 58-kDa cross-linking complex, indicating intermolecular cross-links between these two components.