Fig. 2.

Rarg and Lrh-1 synergistically promote reprogramming. (A) Schematic of the Tet-On reprogramming strategy. (B and C) Rapid activation of endogenous Oct4 (B) or Rex1 (C) by 6F. Expression of 6F for 4 d was sufficient to fully activate endogenous Oct4 (B) or Rex1 (C) to obtain Dox-independent iPSCs. (D) Morphology and GFP expression of Dox-independent colonies from Rex1-GFP reporter MEFs. (Scale bars: 10.0 μm.) (E) Kinetics of the Oct4 locus activation in Oct4-GFP reporter MEFs. The blue or red dots represent percentages of GFP⁺ cells in 6F or 4F transfections, respectively.