Fig. 2.

HSF1 is increased and localized to the nucleus in invasive and in situ breast carcinoma. Photomicrographs of H&E-stained sections and HSF1 IHC of invasive ductal carcinoma (A and B) and the preinvasive lesion, DCIS (C and D). Nonneoplastic breast epithelium is indicated by arrows, and neoplastic cells are indicated by the arrowheads. (E) Representative photomicrographs of tumors from the NHS TMAs that were stained by HSF1 IHC and that were scored as having either no (−), low, or high nuclear HSF1 expression. This example with no nuclear HSF1 expression (−) demonstrates weak immunoreactivity in the cytoplasm. (Scale bar: 20 μM.)