FIG. 2.

Features of the serine-rich repeat protein GspB and the corresponding genetic locus. (A) Restriction map of the M99 chromosomal region spanning gspB. The locations of selected restriction sites in gspB are indicated. H3, HindIII; H2, HincII; Nh, NheI; Ns, NsiI; S, SpeI. (B) Diagram of GspB and truncated derivatives. The number associated with the GspB designation indicates the predicted molecular mass in kilodaltons. GspB194 was engineered with a C-terminal His₆ tag. The hatched sections correspond to srr1 and srr2. wt, wild type.