FIG. 5.

Detection of carbohydrate linked to GspB and other S. gordonii cell wall proteins. The proteins extracted from M99 (lanes 1, 3, and 5) or the gspB mutant strain PS436 (lanes 2, 4, and 6) were separated by electrophoresis through 3 to 8% polyacrylamide gradient gels. The proteins were then transferred to nitrocellulose and either examined for the presence of carbohydrate by using the DIG-glycan detection kit (lanes 1 and 2) or probed with a polyclonal anti-rGspB serum (lanes 3 and 4). To confirm that comparable amounts of total protein were loaded in each lane, gels run in parallel were stained with SYPRO Ruby (lanes 5 and 6).