FIG. 6.

Dependence of GspB194 export on components of the accessory Sec system. GspB194 was detected using an anti-His₆ monoclonal antibody. The upper lanes contain proteins precipitated from 160 μl of the culture medium; the lower lanes were loaded with protoplasts of bacteria in 120 μl of culture. PS498 and PS526 are derivatives of strain PS497 that have mutations in secY2 and secA2, respectively.