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. 2004 Feb;186(3):638–645. doi: 10.1128/JB.186.3.638-645.2004

FIG. 7.

FIG. 7.

SDS-PAGE analyses of truncated derivatives of GspB. The proteins were purified from S. gordonii culture supernatants. The predicted masses (in kilodaltons) of the GspB variants are indicated along the top of the gel. The proteins in lanes 1 to 3 (1 pmol of GspB per lane) underwent Western blot analysis using a polyclonal anti-rGspB serum. The proteins in lanes 4 to 6 (1 pmol per lane) were analyzed for the presence of carbohydrate, using the DIG-glycan detection kit. The proteins in lanes 7 to 9 (4 pmol per lane) were stained with SYPRO Ruby. Note that the proteins in lanes 8 and 9 are refractory to staining with SYPRO Ruby and are thus not readily apparent here. The molecular mass standards correspond to cross-linked multimers of phosphorylase b. Lanes 1, 4, and 7, GspB105; lanes 2, 5, and 8, GspB194; lanes 3, 6, and 9, GspB274.