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Detection of glycosylated GspB in S. gordonii protoplasts. Each lane contained material from protoplasts of cells in a 120-μl culture volume. Lanes 1 to 4, proteins were examined for carbohydrate. Lanes 5 to 8, proteins were subjected to Western blot analysis using the anti-rGspB serum. Lanes 1 and 5, PS426 (GspB286 SecY2−); lanes 2 and 6, PS498 (GspB194 SecY2−); lanes 3 and 7, PS466 (GspB105 SecY2−); lanes 4 and 8, PS436 (GspB−).
