TABLE 1.
Strains and plasmids used in the present study
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Strain | ||
| M99 | S. gordonii parental strain | 22 |
| PS321 | M99 Δ[nss-gtf] | 2 |
| PS426 | M99 secY2::pVA891 | 2 |
| PS436 | M99 gspB::pM995′Bint | 2 |
| PS463 | M99 gspB::pM99midBint; secretes GspB105 | 2 |
| PS465 | M99 gspB::pM993′Bint; secretes GspB274 | 2 |
| PS466 | PS463 secY2::pVA891 | 2 |
| PS469 | M99 secA2::pVA891 | 2 |
| PS497 | M99 gspB::pM99B194His6int; secretes GspB194 | This study |
| PS498 | PS426 gspB::pM99B194His6int | This study |
| PS526 | PS469 gspB::pM99B194His6int | This study |
| Plasmid | ||
| pBluescript | Ampr | Stratagene |
| pVA891 | erm (gram positive) cat tet ori (E. coli) | 12 |
| pEVP3 | cat (gram positive and E. coli) ori (E. coli) | 4 |
| pB194His6int | SpeI-NheI fragment of gspB + His6 in pEVP3 | This study |
| pM995′Bint | HindIII-HincII fragment of gspB in pVA891 | 2 |
| pM99midBint | SpeI fragment of gspB in pEVP3 | 2 |
| pM993′Bint | NheI-NsiI fragment of gspB in pEVP3 | 2 |