TABLE 3.
Monosaccharide analysis of carbohydrates associated with GspB105 and GspB194
| Carbohydrate | Contenta
|
Content/nmol of GspBb
|
||
|---|---|---|---|---|
| GspB105 | GspB194 | GspB105 | GspB194 | |
| GlcNAc | 1.42 | 2.99 | 14 | 30 |
| Glc | 4.05 | 7.26 | 41 | 73 |
| GalNAc | 0.49 | 0.17 | 5 | 2 |
| Gal | 0.77 | 0 | 8 | 0 |
| Rhamnose | 0 | 0 | 0 | 0 |
| Fucose | 0.09 | 0 | Trace (<1) | 0 |
| Mannose | 0 | 0 | 0 | 0 |
| Xylose | 0 | 0 | 0 | 0 |
a
Monosaccharides bound to ∼100 pmol of the purified GspB glycoproteins, with the amount detected in the negative control sample prepared in parallel subtracted.
b
Calculated by dividing the measured amount (in nanomoles) by the amount of each GspB variant analyzed (0.1 nmol).