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. 2011 Oct 24;108(45):18471–18475. doi: 10.1073/pnas.1109680108

Fig. 4.

Fig. 4.

Contextual LTM formation requires mTOR signaling in the dorsal hippocampus in the T286A mutants. WT mice and T286A mutants were trained with massed foreground conditioning. (A) Anisomycin (225 mg/kg) was intraperitoneally (IP) injected immediately after training. Contextual LTM was tested 24 h later. Rapamycin (2.5 μg per side) (B) or actinomycin D (10 ng per side) (C) were bilaterally injected into dorsal hippocampus (IdH) immediately after training and 24 h after conditioning the animals were tested for contextual LTM. (D) Training-induced PSD95 overexpression requires mTOR signaling in the IdH in the T286A mutants. Rapamycin (2.5 μg per side) was bilaterally injected into IdH immediately after conditioning. The ventral and dorsal hippocampi were collected 2 h after training, and lysates were assayed by immunoblotting. *P < 0.05; **P < 0.01; ***P < 0.001.