Fig. 2.
Shared synthetic lethal phenotypes of envC and ftsEX mutants. (A) Cells of MM11/pTB63 (Para::ponB/ftsQAZ) and its ΔenvC, ΔftsEX, or ΔamiA ΔamiB derivatives were grown overnight in M9 arabinose medium supplemented with 5 μg/mL Tet (Tet5) at 37 °C. Following normalization for cell density (OD600 = 2), the resulting cultures were serially diluted (10−1 to 10−6), and 5 μL of each dilution was spotted on the indicated medium. Plates were incubated overnight at 37 °C and photographed. (B) Cells of TU191(attλTU188) [ΔftsEX (Para::ftsEX)] and its ΔenvC or ΔftsP derivatives were processed as in A except growth was in the absence of Tet. (C) Cells of TB140(attλTD25) [ΔenvC (Para::envC)] and its ΔftsP derivative were grown and processed as in B.