Fig. 4.
ANP/GC-A stimulates TRPC3/C6 channel activity in transfected HEK293 cells. Shown are cation currents in HEK coexpressing GC-A and either vector [(A) n = 5] or TRPC3/C6 (B–D) or TRPC3/C6 and PKG I (C and D) (all n = 10–13) and HEK coexpressing GC-AD893A and TRPC3/C6 (D and F) (n = 7). Cells additionally expressed EGFP and were identified by their marked fluorescence. Currents were recorded first under superfusion with bath solution (basal) and afterward during addition of 100 nM ANP. (A, B, D, and F) Current amplitudes in relation to membrane potential of representative cells and mean percentage of changes in response to ANP (C) (*P < 0.05). (E) Effects of ANP on cGMP levels in HEK expressing WT GC-A or GC-AD893A (n = 8). (Inset) Western blotting of GC-A and GC-AD893A membrane expression levels (all 10 μg protein per lane).