Fig. 1.

Physiological responses to nutrient starvation. (A) Survival over time during starvation for methionine (Met), phosphate (Pho), or Leucine (Leu) using the indicated strains. Survival was measured as the ability to form a colony on rich medium plates. (B) Cell cycle arrest, as measured by percentage of cells with no bud (assessed by light microscopy), during starvation for Met, Pho, Leu, or Uracil (Ura) using the indicated strains. Representative time courses are shown, with more comprehensive data provided in Table S1. (C) Glucose consumption (measured as described in Materials and Methods) after biomass (measured by optical density) has reached its plateau during starvation for Met or Leu. Error bars show SD of technical replicates.