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. Author manuscript; available in PMC: 2011 Nov 14.
Published in final edited form as: Biochem Biophys Res Commun. 2010 Sep 15;401(1):58–63. doi: 10.1016/j.bbrc.2010.09.008

Figure 1.

Figure 1

Amide and methyl spectra of the V27A18-60 mutant display residue specific assignment of backbone amine and sidechain methyl groups, respectively. a. 1H-15N tr-HSQC spectrum of 15N-, 13C, and 85% 2H-labeled protein. b. 1H-13C HSQC spectrum of methyl groups of uniformly 15N-, 13C-labeled protein. Both spectra were recorded at 30°C, pH 7.5, and 1H frequency of 600MHz.