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. 2004 Feb;48(2):514–520. doi: 10.1128/AAC.48.2.514-520.2004

FIG. 6.

FIG. 6.

Analysis of culture filtrates from the wild type, the pah1 mutant, and the pah1 and pah2 double mutants of S. clavuligerus by HPLC. Culture filtrates from 96-h cultures grown on soy medium were analyzed by HPLC after derivatization with imidazole. The elution profiles for the pah1 and pah2 double mutant culture (A), the pah1 mutant culture (mutant 5A) (B), and the wild type (C), with detection at 311 nm, are shown. CM, clavaminic acid; C-2-C, clavam-2-carboxylic acid; 2-HMC, 2-hydroxymethylclavam; CA, clavulanic acid.