Table II.
Characterization of fatty acid composition of the mitochondrial inner membrane isolated from shoots of maize seedlings of parental lines and populations grown at 25°C and 14°C
Material | Unsaturation Degreea | Mean Chain Lengthb | Monounsaturated Species | Unsaturated Species | Unsaturated 18-Carbon Species | 18:1/18:0 | Unsat C18/Sat C18c |
---|---|---|---|---|---|---|---|
Δ/mol | Carbon atom no. | mass % | |||||
25°C | |||||||
Parental lines | |||||||
B73 | 0.970 | 17.04 | 4.9 | 49.8 | 49.3 | 1.1 | 12.3 |
IABO78 | 0.980 | 17.05 | 5.1 | 50.2 | 49.6 | 1.1 | 12.5 |
Populations | |||||||
C0 | 0.970 | 17.04 | 4.9 | 49.8 | 49.3 | 1.1 | 12.3 |
C4-L | 0.968 | 17.05 | 5.1 | 49.6 | 49.2 | 1.1 | 11.6 |
C4-H | 0.990 | 17.07 | 5.3 | 51.0 | 50.7 | 1.2 | 12.8 |
Meand | 0.979 | 17.06 | 5.2 | 50.3 | 49.9 | 1.2 | 12.2 |
lsde | 0.011 | 0.02 | ns | 0.6 | 0.6 | 0.1 | 0.5 |
lsdf | nsg | ns | ns | 0.5 | 0.5 | ns | ns |
14°C | |||||||
Parental lines | |||||||
B73 | 1.125 | 17.20 | 6.2 | 57.8 | 57.3 | 1.8 | 17.8 |
IABO78 | 1.163 | 17.23 | 7.6 | 60.1 | 59.3 | 2.3 | 20.5 |
Populations | |||||||
C0 | 1.173 | 17.24 | 6.1 | 59.9 | 59.4 | 1.8 | 19.3 |
C4-L | 1.068 | 17.18 | 5.1 | 54.9 | 54.4 | 0.9 | 10.4 |
C4-H | 1.273 | 17.33 | 9.1 | 65.0 | 64.4 | 3.4 | 25.6 |
Meand | 1.170 | 17.25 | 7.1 | 59.9 | 59.4 | 2.1 | 18.0 |
lsde | 0.020 | 0.02 | 0.3 | 1.3 | 1.3 | 0.2 | 1.2 |
lsdf | ns | ns | 0.3 | ns | ns | 0.2 | 1.0 |
Results are the mean values of four determinations (two experiments and two samples per experiment).
Calculated as follows: Δmol = (1[% monoenes]/100) + (2[% dienes][/100) + (3[% trienes]/100).
Calculated as follows: ∑ (percentage of the fatty acid × its carbon atom number)/100.
Unsat C18/Sat C18, Ratio of unsaturated 18-carbon species and saturated 18-carbon species.
Mean of the two selected populations C4-L and C4-H.
lsd (P ≤ 0.05) for comparing parental lines and/or populations.
lsd (P ≤ 0.05) for comparing the source population C0 with the mean C4-L and C4-H.
ns, Not significant.