Table IV.
Rate of ATP transport in proteoliposomes reconstituted with ANT protein purified from mitochondrial membranes isolated from maize seedlings of populations grown at 25°C and at 14°C
| Population | ATP
Transport
|
|
|---|---|---|
| 25°C | 14°C | |
| nmol 10 min−1 mg−1 protein | ||
| Seedlings grown at 25°C | ||
| C0 | 9109 | 8481 |
| C4-L | 9033 | 8327 |
| C4-H | 9239 | 8604 |
| Meana | 9136 | 8466 |
| lsdb | 84 | 149 |
| lsdc | nsd | ns |
| Seedlings grown at 14°C | ||
| C0 | 7624 | 6648 |
| C4-L | 6824 | 5571 |
| C4-H | 8920 | 7730 |
| Meana | 7872 | 6651 |
| lsdb | 615 | 228 |
| lsdc | ns | ns |
Proteoliposomes were loaded with 20 mm ATP. Transport was initiated by adding 0.1 mm [3H]ATP. Results are the averages of four determinations (two experiments and two samples per experiment).
a
Mean value of the two selected populations C4-L and C4-H.
b
lsd (P ≤ 0.05) for comparing populations.
c
lsd (P ≤ 0.05) for comparing the source population C0 with the mean of C4-L and C4-H.
d
ns, Not significant.