FIG. 2.
Inhibition of E. coli Phe-RS by compound 2. The rate of Phe-tRNA formation was determined at room temperature over 20 min. (A) Competitive binding with respect to Phe. Compound 2 concentrations were as follows: 0 μM (▴), 0.04 μM (▪), and 0.156 μM (⧫). The inset shows results in the form of a Lineweaver-Burk plot (Km for Phe = 1.9 μM; Ki for compound 2 = 21 nM). (B) Noncompetitive binding with respect to ATP. Compound 2 concentrations were as follows: 0 μM (▴), 0.01 μM (▪), and 0.04 μM (⧫).