TABLE 4.
Proteome analysis of B. subtilisa
| Proteinb | Translational induction factorc
|
Function and/or similarityd | |
|---|---|---|---|
| 0.5× MIC, 20 min | 1× MIC, 10 min | ||
| Ald | 2.4 | 2.4 | l-Alanine dehydrogenase |
| Dps | 3.1 | 3.2 | Stress- and starvation-induced gene controlled by sigmaB |
| FbaA | 1.4 | 4.4 | Fructose-1,6-bisphosphate aldolase |
| MinD | 2.8 | 3.6 | Cell-division inhibitor (septum placement) |
| PheS | 2.3 | 1.7 | Phenylalanyl-tRNA synthetase (alpha subunit) |
| Spo0A | 3.2 | 2.4 | Two-component response regulator central for the initiation of sporulation |
| SpoVG | 1.8 | 3.7 | Required for spore cortex synthesis |
| Tpx | 1.8 | 3.7 | Probable thiol peroxidase |
| YceE | 2.7 | >10 | Unknown; similar to tellurium resistance protein |
| YocJ | 2.4 | 2.7 | Unknown; similar to acyl-carrier protein phosphodiesterase |
| YodC | 1.4 | 2.4 | Unknown; similar to nitroreductase |
| YtxH | 1.9 | >10 | Unknown; similar to general stress protein |
| YurL | 2.1 | 2.3 | Unknown; similar to ribokinase |
| YurPe | 10.8 | 8.6 | Unknown; similar to glutamine-fructose-6-phosphate transaminase |
| YvyD | 1.9 | >10 | Unknown; similar to sigma-54 modulating factor of gram-negative bacteria |
Cytoplasmic proteins produced by B. subtilis in response to treatment with compound 1 were separated by two-dimensional gel electrophoresis in the pI range of 4 to 7.
Proteins that were induced more than twofold in both experimental series and that accumulated to amounts sufficient for determination of their identity by peptide mass fingerprinting are listed.
Induction factors represent the ratio of protein synthesis results for the antibiotic-treated versus the control culture. Experiments were performed in duplicate. During each experimental series, two treatment conditions (0.5 times the MIC for 20 min and 1 times the MIC for 10 min) were analyzed. The induction factors correspond to the results of one representative experiment.
Functional annotation according to data from the SubtiList web server (http://genolist.pasteur.fr/SubtiList/).
In the antibiotic-treated as well as the control culture, YurP migrated in several spots on the gels, possibly due to protein modifications. The amount of all YurP spots increased in the presence of the antibiotic. The numbers refer to the YurP spot with the highest induction factor.