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. 2010 Aug 16;1:10.3402/nano.v1i0.5161. doi: 10.3402/nano.v1i0.5161

Fig. 5.

Fig. 5

Fixed cell imaging and simultaneous detection of multiple cellular targets using QD conjugates. (A) Nuclear antigens in the nuclei of human epithelial cells labelled with ANA, anti-human IgG-biotin and QD 630-streptavidin. (B) When normal human IgGs were used, no detectable stain was observed. (C) Simultaneous labelling of nuclear antigens (red) and microtubules (green) using different QD conjugates in a 3T3 cell. (D) Her2 on the surface of SK-BR-3 cells was stained green with mouse anti-Her2 antibody and QD 535-IgG (green). Nuclear antigens were labelled with ANA, anti-human IgG-biotin and QD 630-streptavidin (red). Reprinted with permission from Macmillan Publishers Ltd: Nature Biotechnology (30) copyright 2003.