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. 2011 Oct 24;10:314. doi: 10.1186/1475-2875-10-314

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Copyright ©2011 Moreno-Perez et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Detection of PvRON1 recombinant fragments and the native protein by polyclonal antibodies. (A) Evaluation of anti-PvRON1 polyclonal antibody recognition by Western blot. Lines 1-3 and 8-10 correspond to recognition by pre-immune sera. Lines 4-6 and 11-13 correspond to detection with rabbits' post III sera . Lines 7 and 14 indicate the recognition of purified rPvRON1-a and rPvRON1-c fragments by anti-polyhistidine monoclonal antibody. (B) Detection of the protein in parasite total lysate and DRMs. Lines 1 and 2, parasite lysate in reducing conditions. Lines 3 and 4, parasite lysate in non-reducing conditions. Lines 5-12, DRM fractions treated at 4°C and 37°C. S and P letters represent the supernatant and pellet.