Figure 3. PDR18 transcript levels in yeast cells exposed to 2,4-D-imposed stress.

(A) Comparison of the susceptibility to 2,4-D-induced stress of S. cerevisiae parental strain BY4741 exposed to 0 (◆), 0.3 (■) or 0.45 (▲) mM 2,4-D through cultivation in MM4 liquid medium (pH 3.5). Cells used to prepare the inocula were previously grown in the absence of 2,4-D until mid-exponential phase. Growth curves are representative of at least three independent growth experiments. (B) Comparison of the relative transcript values of PDR18 mRNA/ACT1 mRNA, in cells of parental strain BY4741 during the period of adaptation to 2,4-D by RT–PCR. The PDR18 mRNA value for the control conditions (0 h, unsupplemented medium) was set as 1 and the remaining values were relative values. Values are the means±S.D. for at least three independent experiments.