Fig. 1.
Lymphocyte subsets in 3- and 10-week Mycobacterium bovis strain Calmette-Guerin-induced granulomas. C57BL/6 mice were systemically infected intraperitoneally with BCG. Three and ten weeks post-infection livers were harvested, and granuloma-infiltrating cells were isolated. For P25 experiments, 5×105 dsRed Vβ11+CD4+ cells from the P25 Tg mouse were adoptively transferred into infected mice one week prior to harvest. FACS analysis of CD8+ and CD4+ (top row), P25 (middle row) and NK1.1+ and B220+ (bottom row) populations in liver granulomas. Subset gating scheme shown above is used throughout manuscript. Plots obtained from live cells gate of SSC versus FSC. Plots representative of at least 3–5 independent experiments