Figure 3.

Ethylene production and expression of ACC synthase in carnation ovaries in response to various inducers of ACC synthase activity. Flowers were treated with water as a control (C) or with 25 μm CHX, 50 mm LiCl, 100 μm 2,4-D, or 10 μL L⁻¹ ethylene for 24 h, at which time ovaries were collected for analysis. Ovaries were also collected from pollinated flowers 12 and 24 h after pollination (P12 and P24, respectively) and from senescing flowers 6 d after harvest (6D). A, Ethylene production by the ovaries. Each bar consisted of the average ± se of six ovaries. B, Accumulation of ACC synthase mRNAs in ovaries following treatment. Each lane contained 10 μg of total RNA. Blots were hybridized with the gene-specific ACC synthase probes DCACS1–3′, DCACS2–3′, and DCACS3–3′ and with an rRNA probe. ACC synthase blots were exposed to film for 5 d.