Table 1. Primer and Probes used in Real Time PCR.
| Gene symbol | Gene name | Primer sequence (5’>3’) | Probe sequence (5’>3’) |
|---|---|---|---|
| SPP1 | osteopontin | F-GCCAGTTGCAGCCTTCTCA | CCAAACGCCGACCAAGGAAAACTCAC |
| R-AAAAGCAAATCACTGCAATTCTCA | |||
| COL1A1 | collagen type I alpha1 |
F-TAGGGTCTAGACATGTTCAGCTTTGT | CCTCTTAGCGGCCACCGCCCT |
| R-GTGATTGGTGGGATGTCTTCGT | |||
| RUNX2 | runt-related transcription factor 2 |
F-TCTACCACCCCGCTGTCTTC | ACTGGGCTTCCTGCCATCACCGA |
| R-TGGCAGTGTCATCATCTGAAATG | |||
| ALPL | alkaline phospatasi | F-CCGTGGCAACTCTATCTTTGG | CCATGCTGAGTGACACAGACAAGAAGCC |
| R-CAGGCCCATTGCCATACAG | |||
| COL3A1 | collagen, type III, alpha 1 |
F-CCCACTATTATTTTGGCACAACAG | ATGTTCCCATCTTGGTCAGTCCTATGCG |
| R-AACGGATCCTGAGTCACAGACA | |||
| BGLAP | osteocalcin | F-CCCTCCTGCTTGGACACAAA | CCTTTGCTGGACTCTGCACCGCTG |
| R-CACACTCCTCGCCCTATTGG | |||
| CD105 | endoglin | F-TCATCACCACAGCGGAAAAA | TGCACTGCCTCAACATGGACAGCCT |
| R-GGTAGAGGCCCAGCTGGAA | |||
| FOSL1 | FOS-like antigen 1 | F-CGCGAGCGGAACAAGCT | ACTTCCTGCAGGCGGAGACTGACAAAC |
| R-GCAGCCCAGATTTCTCATCTTC | |||
| SP7 | osterix | F-ACTCACACCCGGGAGAAGAA | TCACCTGCCTGCTCTGCTCCAAGC |
| R-GGTGGTCGCTTCGGGTAAA | |||
| RPL13A | ribosomal protein L13 |
F-AAAGCGGATGGTGGTTCCT | CTGCCCTCAAGGTCGTGCGTCTG |
| R-GCCCCAGATAGGCAAACTTTC |