Figure 1.

Alignments of the deduced amino acid sequences of the potato GA 20-oxidase clones StGA20ox1, StGA20ox2, and StGA20ox3 with those of pea (accession no. PsU58830), spinach (accession no. SoU33330), pumpkin (Cm20ox; accession no. X73314), and Arabidopsis (At2301; accession no. X83379). Identical residues are boxed in black; similar residues are shaded in gray. The conserved LPWKET and NYYPXCQKP regions thought to be involved in binding the GA substrate and the 2-oxoglutarate cosubstrate are underlined. Conserved H and D residues involved in the binding of Fe²⁺ are also indicated (*). Alignments were made using the PileUp and PrettyBox programs of the Genetics Computer Group.