Figure 6.

Diurnal variation of levels of StGA20ox2 and StGA20ox3 transcripts in leaves. Plants were grown in short days (SD) or short days with a night break (SD + NB) for 3 weeks. RNA blots were hybridized to probes StGA20ox2 and StGA20ox3, and subsequently to the ribosomal S4 protein probe for normalization. Densitometric scanning of the film quantified the levels of StGA20ox2 and StGA20ox3 mRNAs. The graphs represent the levels estimated for both transcripts compared with the strongest hybridization signal (short days, 23.5 h, in RNA blots hybridized with probe StGA200ox1), to which we assigned an arbitrary value of 100. Values correspond to approximately the levels of the respective mRNAs, as probes of about the same size and specific activities were used for hybridization. The experiment was repeated twice.