Table 4.

Correlations Between Evolutionary Rates and CpG O/E.

	CpG O/E	P Value	CpG O/E|GC Content	P Value
Comparison among Nasonia sibling species
dN	0.117	<10−16	0.153	<10−16
dS	0.218	<10−16	0.157	<10−16
dI	0.226	<10−16	0.130	<10−16
dN/dS	−0.002	NS	−0.001	NS
Nasonia–Apis comparisona
dN	0.066	<10−5	0.099	<10−11

NOTE.—Comparisons in two timescales (between sibling species of Nasonia and between Nasonia and honeybee) provide support for a negative relationship between the level of DNA methylation and rates of nucleotide substitution.

^aBecause synonymous sites are saturated for most genes, we only analyze nonsynonymous substitutions.