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. 2004 Feb;48(2):612–614. doi: 10.1128/AAC.48.2.612-614.2004

FIG. 1.

FIG. 1.

Mapping of transcription initiation site of A. baumannii RAN ampC by primer extension analysis. DNA sequencing fluorograms generated with indodicarbocyanine-labeled primer are shown, with the peaks corresponding to the nucleotides indicated. (A) DNA sequence of template strand upstream of ampC initiation codon, starting at the initiation codon (CAT). (B) T sequencing reaction products and primer extension product. The transcription start site is indicated by the vertical arrow. (C) Nucleotide sequence of the regulatory region (300 bp) upstream of the ampC initiation codon (boxed). TTAGAA (−35)-N16-TTATTT (−10) upstream of the transcription start site are shown in bold. Additional −35 and −10 hexamers are underlined. The coincident G demarcating the start of the homologous region is shown in bold. The ribosome-binding site (RBS) is indicated in italics.