Fig. 2.

G-CSFR and Prdx4 interaction and colocalization. (A) HeLa cells ectopically expressing wild-type G-CSFR were deprived of growth factor for 4 hours. Surface membrane G-CSFRs were labelled with anti-G-CSFR antibody prior to stimulation with G-CSF for different times. Cells were permeabilized, fixed and stained with anti-Prdx4 antibody, followed by secondary anti-rabbit Cy3 and anti-mouse Cy5 antibodies, and then analysed by CLSM. (B) Colocalization of endocytosed G-CSFR (arrows) with Grp94-stained ER (left) and with ERGIC-53-stained ERGIC (right) 30 minutes after ligand stimulation. Experimental conditions were the same as for A.