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. 2004 Feb;48(2):608–611. doi: 10.1128/AAC.48.2.608-611.2004

FIG. 1.

FIG. 1.

Effects of K-Glu on ParC Aα4 Topo IV-catalyzed decatenation activity. The kDNA decatenation assay was performed in the presence of either 10 fmol (as tetramer) of the wild-type Topo IV (lanes 2 to 7) or 40 fmol (as tetramer) of ParC Aα4 Topo IV (lanes 8 to 13), 0.3 μg of kDNA, and the indicated concentrations of K-Glu. Relative amounts (100% = 0.3 μg) of decatenated kDNA were as follows: lane 1, 0%; lane 2, 12%; lane 3, 64%; lane 4, 58%; lane 5, 58%; lane 6, 5%; lane 7, 0%; lane 8, 3%; lane 9, 4%; lane 10, 6%; lane 11, 57%; lane 12, 49%; and lane 13, 0%. wt, wild-type Topo IV; Aa4, ParC Aα4 Topo IV.