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Ternary complexes formed with ParC Aα4 Topo IV are more stable than those formed with the wild-type Topo IV. The DNA cleavage was performed in the presence of 20 fmol (as molecule) of 32P-labeled linear pBR322 DNA; 50 μM norfloxacin; 100 fmol (as tetramer) of Topo IV (lanes 2 to 6), gyrase (lanes 7 to 11), or ParC Aα4 Topo IV (lanes 12 to 16); and the indicated concentrations of K-Glu. Aa4, ParC Aα4 Topo IV.
